GoScript Reverse Transcription Mix, Oligo(dT) from Promega

Product Specs
ItemGoScript Reverse Transcription Mix, Oligo(dT)
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA2791
Quantity100 reactions

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancerJournal of Experimental & Clinical Cancer ResearchOctober 20, 2023Pingping Su, Xiaodan Mao, Jincheng Ma, Lixiang Huang, Lirui Yu, Shuting Tang, Mingzhi Zhuang, Zhonglei Lu, Kelvin Stefan Osafo, Yuan Ren, et al.alcohol. Wash RNA with 75% ethanol. First Strand cDNA Synthesis and preparation of PCR Master Mix (#A2791, Promega, Madison, USA). PCR amplification was performed using Eastep QPCR Master Mix (#LS2062, Promega,

(2) PARP-1 improves leukemia outcomes by inducing parthanatos during chemotherapyCell reports. MedicineSeptember 19, 2023Maru B, Messikommer A, Huang L, Seipel K, Kovecses O, Valk PJM, Theocharides APA, Mercier FE, Pabst T, McKeague M, et al.Monarch Total RNA Miniprep Kit,New England Biolabs,Cat# T2010S GoScript™ Reverse Transcription Mix,Promega,Cat# A2791 2x TaqMan™ Fast Advanced Master Mix,Thermo Fisher Scientific,Cat# 4444556 20x PARP1 TaqMan Expression

(3) Ribonucleotide synthesis by NME6 fuels mitochondrial gene expressionThe EMBO journalSeptember 18, 2023Grotehans N, McGarry L, Nolte H, Xavier V, Kroker M, Narbona-Pérez ÁJ, Deshwal S, Giavalisco P, Langer T, MacVicar T.NucleoSpin RNA,Machery‐Nagel,740955.250 DNeasy Blood and Tissue Kit,Qiagen,69506 GoScript Reverse Transcription,Promega,A2791 Bio‐Rad Protein Assay Kit,Bio‐Rad,5000001 Pierce Protein Assay Reagent,Thermo Fisher Scientific,22660 FluorSave Reagent

(4) Time-resolved proteomic analyses of senescence highlight metabolic rewiring of mitochondriaLife science allianceSeptember 1, 2023Kim JY, Atanassov I, Dethloff F, Kroczek L, Langer T.cells (740955; Macherey-Nagel) and subjected to cDNA synthesis with oligo(dT) reverse transcriptase (A2791; Promega) according to the manufacturer's protocol. Target mRNA levels were quantified by ΔΔCt values

(5) ER‐mitochondria contacts and cholesterol metabolism are disrupted by disease‐associated tau proteinEMBO reportsAugust 3, 2023Leonora Szabo, Nadia Cummins, Paolo Paganetti, Alex Odermatt, Andreas Papassotiropoulos, Celeste Karch, Jürgen Götz, Anne Eckert, Amandine Grimm(RNA),Takara,631450 RNeasy Mini Kit (250),Qiagen,74106 "GoScript™ Reverse Transcription Mix, Oligo(dT)",Promega,A2791 GoTaq® Probe qPCR Master Mix,Promega,A6102 MycoAlert® PLUS Mycoplasma Detection Kit,Lonza,LT07‐701 Oligonucleotides,Oligonucleotides,Oligonucleotides AllStars Negative Control siRNA

(6) Local Administration of Minocycline Improves Nerve Regeneration in Two Rat Nerve Injury ModelsInternational journal of molecular sciencesJuly 28, 2023Guillemot-Legris O, Girmahun G, Shipley RJ, Phillips JB.instructions. cDNA was synthesised using the GoScript Reverse Transcriptase kit (Promega, Southampton, UK. ref: A2791). RT-qPCR was performed with a QuantStudio 3 instrument (Applied Biosystems, Waltham, MA, USA) and analysed

(7) Elafin as a Prognostic Marker in Esophageal Squamous Cell Carcinoma: A Pilot Study Using Three-Dimensional Imaging and Genomic ProfilingCancers (Basel)July 27, 2023Wei-Chung Chen, Chun-Chieh Wu, Yu-Peng Liu, Guan-Yu Zhuo, Yao-Kuang Wang, Yi-Hsun Chen, Chu-Chih Chen, Yin-Han Wang, Ming-Tsang Wu, I-Chen Wu74404; QIAGEN, Venlo, The Netherlands) and cDNA was synthesized by GoScript™ Reverse Transcriptase (Cat. A2791, Promega, Madison, WI, USA). Elafin expression was quantified using Taqman gene expression assay reagents

(8) Aqueous Humor TGF-β2 and Its Association With Intraocular Pressure in a Naturally Occurring Large Animal Model of GlaucomaInvestigative ophthalmology & visual scienceJuly 3, 2023Oikawa K, Torne O, Sun D, Moon AKB, Kiland JA, Trane RM, McLellan GJ.The RNAs were reverse-transcribed with GoScript Reverse Transcriptase and oligo-dT primers (catalog # A2791; Promega, Madison, WI, USA). TaqMan probes were designed and generated to target feline TGFB2 (Forward:

(9) Defects in Glutathione System in an Animal Model of Amyotrophic Lateral SclerosisAntioxidants (Basel)April 27, 2023Franziska T. Wunsch, Nils Metzler-Nolte, Carsten Theiss, Veronika Matschkecomplementary desoxyribonucleic acid (cDNA) using the GoScript™ Reverse Transcription Mix, Oligo(dT) (#A2791, Promega, Madison, WI, USA) according to the manufacturer’s instructions. The quantitative real-time polymerase chain reaction

(10) Spermidine Rescues Bioenergetic and Mitophagy Deficits Induced by Disease-Associated Tau ProteinInternational Journal of Molecular SciencesMarch 10, 2023Lauren H. Fairley, Imane Lejri, Amandine Grimm, Anne Eckertinstructions. cDNA synthesis was carried out using the GoScript™ Reverse Transcription Kit (Promega, A2791) in an RNase-free environment. Only RNA samples with 260/280 nm of 2.0 ± 0.2 were used. Real-time PCR,

(11) The global downregulation of protein synthesis observed during hepatogenic maturation is associated with a decrease in TOP mRNA translationStem Cell ReportsJanuary 1, 2023Marino Caruso, Sébastien Meurant, Damien Detraux, Amandine Mathieu, Manon Gilson, Marc Dieu, Antoine Fattaccioli, Catherine Demazy, Mustapha Najimi, Etienne Sokal, et al.extractions and reverse transcription (RT) using GoScript reverse transcriptase with random primers (Promega A2791). A ReliaPrep RNA Miniprep System (Promega Z6010) was used for all RNA extractions by mixing samples

(12) Serum BRD2 autoantibody in hepatocellular carcinoma and its detection using mimotope peptide‑conjugated BSAInternational journal of oncologyDecember 1, 2022Heo CK, Lim WH, Park I, Choi YS, Lim KJ, Cho EW.(RT) was performed using the GoScript™ Reverse Transcription Mix kit (Promega Corporation, cat. no. A2791). PCR was carried out using 2X Taq PCR Pre-Mix (BIOFACT, cat. no. ST302-10h) using a Verti 96 well Thermal

(13) Multi-omics analysis based on 3D-bioprinted models innovates therapeutic target discovery of osteosarcomaBioactive MaterialsDecember 1, 2022Yixuan Lin, Yiqi Yang, Kai Yuan, Shengbing Yang, Shuhong Zhang, Hanjun Li, Tingting Tang(Carlsbad, CA, USA). A reverse transcription kit was purchased from Promega (GoScript™ transcription kit, A2791, Mannheim, GE). SYBR Green qPCR Master Mix and cell counting kit-8 (CCK-8) assay were purchased from

(14) The impact of macrophages on endothelial cells is potentiated by cycling hypoxia: Enhanced tumor inflammation and metastasisFrontiers in OncologySeptember 28, 2022Victor Delprat, Camille Huart, Olivier Feron, Fabrice Soncin, Carine MichielsIMPLEN), and 2 µg of RNA was reverse-transcribed using the GoScript Reverse Transcription Mix, Oligo(dT) (#A2791, Promega). qPCR was performed as in (9). RPL13A and β-2-microglobulin genes were used for normalization

(15) Interference of Dihydrocoumarin with Hormone Transduction and Phenylpropanoid Biosynthesis Inhibits Barnyardgrass (Echinochloa crus-galli) Root GrowthPlants (Basel)September 26, 2022Haona Yang, Shangfeng Zhou, Lamei Wu, Lifeng WangVerification The extracted total RNA was reverse-transcribed using the reverse transcription kit (A2791, Promega, Madison, WI, USA), and the entire 20-μL reaction system was adopted in this project. A real-time

(16) Lost in translation: Molecular basis of reduced flower coloration in a self-pollinated monkeyflower ( Mimulus ) speciesScience AdvancesSeptember 16, 2022Mei Liang, Caitlin E. Foster, Yao-Wu YuanSigma-Aldrich), and cDNA was synthesized from 500 ng of total RNA with GoScript reverse transcriptase (A2791, Promega) and then diluted fourfold before PCR. (q)RT-PCR was carried out as previously described (22).

(17) The Oligodendrocyte Transcription Factor 2 OLIG2 regulates transcriptional repression during myelinogenesis in rodentsNature communicationsMarch 17, 2022Zhang K, Chen S, Yang Q, Guo S, Chen Q, Liu Z, Li L, Jiang M, Li H, Hu J, et al.Reverse transcription was performed using the GoScript™ Reverse Transcription System (Promega, Cat# A2791). Then cDNA was amplified by real-time quantitative RT-PCR using SYBR Green (Vazyme, Cat# Q121-02) reagent.

(18) Comparative genomics analysis of bHLH genes in cucurbits identifies a novel gene regulating cucurbitacin biosynthesisHorticulture ResearchFebruary 19, 2022Yuanchao Xu, Huimin Zhang, Yang Zhong, Naiyu Jiang, Xiaoyun Zhong, Qiqi Zhang, Sen Chai, Hongbo Li, Zhonghua Zhangsamples of 1 μg were reverse-transcribed using a GoScript™ Reverse Transcription Mix, Oligo(dT) (Promega, A2791, USA) according to the instruction manual. Then a quantitative PCR assay was performed on an ABI 7900

(19) Lipid reprogramming induced by the TFEB-ERRα axis enhanced membrane fluidity to promote EC progressionJournal of experimental & clinical cancer research : CRJanuary 19, 2022Mao X, Lei H, Yi T, Su P, Tang S, Tong Y, Dong B, Ruan G, Mustea A, Sehouli J, et al.reverse transcription on 500 ng of total RNA with GoScriptTM Reverse Transcription Mix, Oligo (dT) (#A2791, Promega, Madison, Wis, USA), quantitative PCR amplification was performed on the LightCycler 480 (Roche,

(20) Scrapie-Responsive Gene 1 Promotes Chondrogenic Differentiation of Umbilical Cord Mesenchymal Stem Cells via Wnt5aStem cells internationalJanuary 1, 2022Zhou Y, Zheng T, Li L, Guo Y, Xiao J, Wang J, Du Z, Gao H, Tang W, Yang L, et al.Promega, USA), and total RNA was reverse transcribed into cDNA using a GoScript Reverse Transcription Mix (A2791, Promega, USA). We performed qRT-PCR using 1 μg of cDNA with a SsoFast EvaGreen® Supermix Kit (1725201,

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